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Topic: Salvia Divinorum Genetic Exchange program.  (Read 3667 times)
 
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Cakes
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« Reply #15 on: December 16, 2007, 04:05:24 pm »

maybe you need a ladder.

Colchicine IS used on many plants, it actually makes new species by definition. and it is used not just on cannabis and it CAN affect the active chems.

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Overall, treated plants showed a 166-250% increase in THC with respect to controls and a decrease of CBD (30-33%) and CBN (39-65%). CBD (cannabidiol) and CBN (cannabinol) are cannabinoids involved in the biosynthesis and degradation of THC. THC levels in the control plants were very low (less than 1%). Possibly colchicine or the resulting polyploidy interferes with cannabinoid biogenesis to favor THC.

In addition to overexpressing the gene(s) or transferring them, we wonder about grafting.

but presently, in cannabis, besides varying by strain, I have also seen that chem effects were affected by the light waves used to grow the plant (red and UV) and the timing of the harvest (couchlock or not) and by the method of ingestion and by the fertilizers used (chitlin).

re ferts>with other plants, alkaloid contents can have different ratios of constituents and have higher overall contents if they are 'hard grown'.
and there has been great luck in producing alkaloids by supplementing the plant with the alkaloid's precursors. cacti are good for this. and lately i was reading about success with growing mushrooms on mimosa bark.
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Paradoxic
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« Reply #16 on: December 16, 2007, 05:57:40 pm »

Thats very interesting. However, it does seem possible that there might be certain risks associated with this kind of manipulation. Since chemical concentrations could be altered significantly in the plant it seems conceivable that a compound occurring at a relatively safe level could become dangerously concentrated. Maybe thats being paranoid, but who knows when you screw with nature, heheh.

Anyways it would be interesting to see what colchicine would do to Salvia. It was interesting that you say growing mushrooms on mimosa bark provides precursors for psilocybin and such. I never knew that plants could actually incorporate chemicals like that.
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« Reply #17 on: December 16, 2007, 07:12:03 pm »

I think with cannabis,you have thousands and thousands of seeds to play with, as colchicine treatments have high mortality rate ( this could have changed, since I "did" my research)  Not sure if salvia could withstand the dip..but you never know!!! Worth a try.... Grin Grin

Lunas were found at the base of some H and W's in Hawaii, by siebert...they "supposedly" taste smoother  when quidded.....(taste bitter to me!!!)  I  havn't tested strength...all the salvias, as far as I know, have one of the highest concentrations of active chemicals of any plant, perhaps excluding poisons....so from my perspective, the best use of genetic mutations would be for
 A. sunlight or heat tolerance and
B. letting salvinorin A be assimilated through the stomach.... Shocked Shocked Shocked Shocked
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« Reply #18 on: December 16, 2007, 07:26:36 pm »

With Salvia's already low germination rate... I don't think a colchicine treatment would do you any good at all. Salvia seeds can barely take a sterilization in H2O2 before germination, I doubt they could stand up to this harsh carcinogen.

However... a Single seed raised polyploid individual could potentially create hundreds if not thousands of clones over time... A worthwhile investment if you're setting your own seeds!

When I induce flowering this winter, I'll consider trying this.
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« Reply #19 on: December 25, 2007, 01:44:58 am »

seems like you lose as much as your gain.....I don't think salvia could withstand that sort of treatment, it can barely handle the gentlest treatments, let alone that one...anyways, Siebert(visited my patch) believes Luna is polyploid already. Its stomata are 2x larger, leaves are thicker and stronger.  In my experience though, it seems to grow 1/2 as fast....gets swamped over by other plants. 

Young plants leaf structure, (except the Lunas) for me, hasn't been much help in determining what the adult will be like.  I have way too many variables, growing half  outside, half inside, some in pots for recovery, some not, some in the  ground etc.  Roll Eyes  I'm pretty lazy about things sometimes. Heres a nice luna flower to redeem my flakiness. I think thats a Luna to left as well, serrates are a good indicator, in addition to  rounder shape of leaves.




Wow, she is a beauty.
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« Reply #20 on: January 05, 2008, 12:54:34 pm »

I am new here but have been growing Salvia D for about 2 years now.  I have some strains that I picked up from Theatrum Botanicum that I can offer to trade/share.  I would love to get in on the conservation and breeding.  I have been breeding other tropical plants for a few years with decent success.  I have tried crosses with my strains but have not had any luck.

I will take some pics of my blooming plants and post them shortly.

Do you guys know if Theatrum Botanicum is a reliable source??  I have luna from there but I don't notice a huge difference.  Maybe they just re-label the same plants and sell them?Huh
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« Reply #21 on: January 07, 2008, 02:47:49 am »

Hey guys.. i'm a noob when it comes to salvia and growing in general... but how do these new strains come about? I know the very first salvia plant that were brought to the wester world was a hoffman right? so aren't they all just a clone of  hoffman? how do these new strains come about? and how do they differ from each other in terms of the effects during trips?
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« Reply #22 on: January 07, 2008, 01:49:31 pm »

Hey guys.. i'm a noob when it comes to salvia and growing in general... but how do these new strains come about? I know the very first salvia plant that were brought to the wester world was a hoffman right? so aren't they all just a clone of  hoffman? how do these new strains come about? and how do they differ from each other in terms of the effects during trips?

a very few amount of people have been able to get Salvia to set seed. it has a low germination rate ect. Pretty much all of these new strains comming out are from seedlings produced from these people(our seamac being one of them) these seedlings often have genetic variations, like different shaped leaves, stronger varigation on the leaves (saw tooth) ect. It would be cool if it was possible over the next 20 years to maintain this seeding of new plants, allong with re-crossing the new plants, hand hopefully getting better germination results by more genetic range (Luna c+ aurora plan ) crossed with a (aquarius + pheonix plant) = 4th generation of sexy. feel me?
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« Reply #23 on: January 07, 2008, 02:45:39 pm »

so these plants just mutated by themselves? or are some them the result of a crossbreed between two varieties? like you said luna x hoffa...etc... how do you crossbreed plants?

oh antoher question.... what's better hydro or soil? i want to get a cutting of the salvia plant and grow it and develop a relationship with it prior to consumption..... but i wonder if the spirit of the plant will be lost or be differernt since there's no soil (earth) also with no natural sunlight (if you're artificial lights) i wonder if the result will be different.

ps. does anyone havea  cutting they could share with me by any chance? i'm in mass.
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« Reply #24 on: January 07, 2008, 05:16:24 pm »

salvia divinorum rarely sets seed, and normally salvia is obtained from a cutting. so most of the plants we see are from the EXACT same plants, brett blosser or albert hoffman brought back from oaxaca mexico (pronounced wacaca). this is because it IS the same plant. so now we see, when a salvia divinorum sets a seed an it is viable and germinates, the plants sometimes are different than the mother plant the seeds came from, this is the only way to create variation in these plants. i hope that helps you.

p.s salvia does AMAZING things in hydro systems. i wish i did have a cutting for you (my plants not growing very much right now), its an amazing specimen of plant, and has done amazing things for me
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« Reply #25 on: January 12, 2008, 02:24:29 pm »

so these plants just mutated by themselves? or are some them the result of a crossbreed between two varieties? like you said luna x hoffa...etc... how do you crossbreed plants?

When plants reproduce sexually, new phenotypes/genotypes are often produced. Sexual reproduction is one of the reasons that our planet is so diverse today, as without it, new genotypes would be created solely through mutation. Crossbreeding strains results in a plant that's phenotypically similar to both of the parent plants, often with their own traits. These new traits are what makes the plants a "different strain."
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« Reply #26 on: January 12, 2008, 02:28:19 pm »

and how do they differ from each other in terms of the effects during trips?
There is no notable trip variation among strains as far as I know, its just the salvinorin A that gives you the experience. Salvinorin A is so overpowering you wouldn't notice the changes due to other partially active chemicals (there probably are some) in the plant that might be at different concentrations from strain to strain. I can see why you'd think a different strain would give a different effect because it happens in MJ, but since THC isnt that strong the other active chemicals in the plant can actually significantly alter the experience...at least thats what I think.
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« Reply #27 on: January 12, 2008, 08:20:07 pm »

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...brett blosser or albert hoffman brought back from oaxaca mexico.. (pronounced wacaca).

salvia should never be associated with caca. the X sounds like an H and the O is pronouced as W. wahaca 
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« Reply #28 on: January 13, 2008, 02:51:34 pm »

typo, sorry. Smiley i had spoke with my spanish professor about it once, it is known for Many things, not just salvia. its a very rich area as far as culture
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« Reply #29 on: January 13, 2008, 06:35:33 pm »

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...brett blosser or albert hoffman brought back from oaxaca mexico.. (pronounced wacaca).

salvia should never be associated with caca.
LOL!  But yeah its definitely got an "ha" sound in it...the "xa" acts more as an "ha".
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