Whats the resin content of your leaf?

[Defenestrate]

So, In a quest to get some actual hard data about salvia, I'll need some help from anyone with some leaf and some time. The goal here is to find the average amount of tar, or resin, you can extract from plain leaf. My idea is to have anyone who wants to participate extract some leaf, under some specific conditions:

Conditions:

-You state what strain, if you know, the leaf came from
-Room temperature does not go above 77*F, or below 73*F during extraction
-Acetone be used for the extraction
-The acetone be at room temperature
-Leafs are to be ground to a fine powder before extraction
-Three extractions are to be done
-All extracts are evaporated in one dish
-The dish be weighed prior to the extract being added, and weighed after ALL solvent has evaporated, so we can find the weight of all the resin, including the stuff that would stick to the dish after scraping it up
-Your results be posted in grams per kilograms. IE, If I extracted one kilo of leaf, and got ten grams of resin, that would be reported as ten grams per kilogram. I know that not everyone, myself included, has that much leaf lying around, so any amount of leaf may be used so long as you post the amount of leaf you used, and the amount of resin you got.

This is just a draft, and as such, input would be appreciated greatly. Finding the resin content of samples from all over the place would be cool, as then we can compare the growth conditions of the plants to their resin content and overall potency to find what produces the best salvia.

And hey, in the end, atleast you'll have some extract, right? 

[Arcygenical]

I *might* be ordering another kilo soon. if so, I'll add to this.

[Defenestrate]

Cool. Any suggestions for the extraction procedure? I don't want to leave anything out.

[Arcygenical]

Nope, that looks good to me really. I'll weigh it before I purify it with Naphtha, and of course, after.

[Defenestrate]

Awesome. I should be extracting in the next 2-3 weeks, so we should have some numbers soon. Perhaps this should be another thing to put in with the growlogs, so we can match up resin content and potency with different conditions and plant age?

[Arcygenical]

I think we should go with LCMS analysis rather than resin content analysis for something like that... 

[Defenestrate]

Indeed, resin content to potency and growth conditions wouldn't yield very conclusive results for much of anything, comparably. I think the focus now should be to just get the data to work with and then plan from there, eh?

[Arcygenical]

Yep!

If I sell my salvia stock, hopefully relatively soon, I should have another kilo in the mail the same day. :p

[DrYRHead]

One had found room temp acetone to work just fine.
It made for a 20X SD resin.

91% IPA(iso-propyl alcohol) worked, but the IPA made for a 15X resin.
Such that more non-active stuff came through.

Either resin was redissolved into ethanol to make a tincture.

[Arcygenical]

I bought 2 kilos from 2 different vendors.

My first kilo extraction is just being dried now.

I'm going to add 50ml of naphtha to a beaker, measure the weight of that, add it to my resin extraction, mix it, re-transfer to the same beaker, and re-weigh.

Gimme 5m

[Arcygenical]

Kilo1: 4.9g
Kilo2: 5.7g
kilo3: 7.1g (wow!)

I'm keeping everything seperate too, to see which has the most salvinorin A.

[Synchronium]

You should specify a proper method if the results are to mean anything. Acetone strips a lot of shit out the leaves really fast, so if two people differ in the time the leaf is in acetone, the results will be quite a bit different.

The more care + detail you put into your method, the easier it will be to replicate and the better your results will be.